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qam cyt 1 1 mouse inflammation microarray kit (RayBiotech inc)

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RayBiotech inc qam cyt 1 1 mouse inflammation microarray kit
Qam Cyt 1 1 Mouse Inflammation Microarray Kit, supplied by RayBiotech inc, used in various techniques. Bioz Stars score: 93/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+cytokine+microarray+kit/Mouse+Cytokine+Array+Q1/pm39699275-62-19-24
Average 93 stars, based on 16 article reviews

qam cyt 1 1 mouse inflammation microarray kit - by Bioz Stars, 2026-10

93/100 stars

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Protein Array:

Article Title: Tumor-associated macrophages favor C26 murine colon carcinoma cell proliferation in an oxidative stress-dependent manner.
Article Snippet: .. To assess the link between proliferative effects of TAMs on C26 cells and TAM-driven angiogenesis, 24 proteins involved in this process were screened by protein array (RayBio Mouse Angiogenic Cytokine Antibody Array kit; RayBiotech, Inc.) in C26 cell lysates, and also in lysates obtained after C26 cell cultivation with TAMs for 48 h. The results presented in Table I show an overall enhancement of the production of the angiogenic proteins with 112% in the cell lysates obtained under co-culture conditions compared to their production in C26 cells cultivated alone. ..

Article Title: Dietary Apigenin Suppresses IgE and Inflammatory Cytokines Production in C57BL/6N Mice
Article Snippet: Flavonoids ubiquitously exist in plants, vegetables, fruits, and teas.. We evaluated the effect of dietary apigenin, one of the well-known flavonoids, on the immune system in C57BL/6N mice.. Mice were fed experimental diets containing apigenin for 2 weeks.

Ab Array:

Article Title: PPAR-γ/NF-kB/AQP3 axis in M2 macrophage orchestrates lung adenocarcinoma progression by upregulating IL-6.
Article Snippet: .. Cytokine-expression profile analysis A human cytokine antibody array kit (AAM-CYT-1-2, Ray Biotech, USA) was used to detect 23 mouse cytokines simultaneously in culture supernatants from RAW264.7-derived M2 macrophages. ..

Article Title: The attenuating effects of plumbagin on pro-inflammatory cytokine expression in LPS-activated BV-2 microglial cells
Article Snippet: Penicillin/streptomycin and DPBS were obtained from Atlanta Biologicals (Atlanta, GA, USA). .. Mouse Cytokine Antibody Array Kit (Cat # AAM-CYT-1000) and ELISA kits were purchased from RayBiotech (Norcross GA, USA). .. Anti-iNOS antibody (Ab 49999) and goat anti-mouse Alexa Fluor ® 488 (ab 150117) were obtained from Abcam (Cambridge, MA, USA).

Article Title: PPAR-γ/NF-kB/AQP3 axis in M2 macrophage orchestrates lung adenocarcinoma progression by upregulating IL-6
Article Snippet: .. A human cytokine antibody array kit (AAM-CYT-1-2, Ray Biotech, USA) was used to detect 23 mouse cytokines simultaneously in culture supernatants from RAW264.7-derived M2 macrophages. ..

Article Title: TREM2 Agonism with a Monoclonal Antibody Attenuates Tau Pathology and Neurodegeneration.
Article Snippet: .. Blood was examined for hematological and biochemical parameters such as cytokines (RayBio® Mouse Cytokine Antibody Array Kit, Peachtree Corners, GA, USA), human IgG4 (ELISA assay kit, Cat #: BMS2095 Invitrogen, Carlsbad, CA, USA), and soluble TREM2 levels (ELISA assay kit, Cat #: #LS-F7884, LifeSpan BioSciences Inc., Seattle, WA, USA). .. Protein levels in brain lysates were detected using a protein array glass chip with a fluorescence detection assay kit (RayBio®) following manufacturer protocol.

Enzyme-linked Immunosorbent Assay:

Concentration Assay:

Article Title: Pediococcus acidilactici Y01 reduces HFD-induced obesity via altering gut microbiota and metabolomic profiles and modulating adipose tissue macrophage M1/M2 polarization.
Article Snippet: Obesity-related metabolic syndrome is intimately associated with infiltrated adipose tissue macrophages (ATMs), gut microbiota, and metabolic disorders.. Pediococcus acidilactici holds the potential to mitigate obesity; however, there exist strain-specific functionalities and diverse mechanisms, which deserve extensive exploration.. This study aims to explore the potential of P. acidilactici Y01, isolated from traditional sour whey, in alleviating HFD-induced metabolic syndrome in mice and elucidating its underlying mechanism.

Microarray:

other:

Article Title: Dendrobium huoshanense stem polysaccharide ameliorates rheumatoid arthritis in mice via inhibition of inflammatory signaling pathways.
Article Snippet: The present study explored the beneficial effect of Dendrobium huoshanense stem polysaccharide (cDHPS) after oral administration on rheumatoid arthritis (RA) using type II collagen-induced arthritis (CIA) mouse model.. It was found that cDHPS effectively alleviated joint swelling, synovial hyperplasia, pannus formation, cartilage erosion and bone destruction in CIA mice.. Concurrently, cDHPS remodeled the balance of Th17 and regulatory T cells, reduced the secretion of pro-inflammatory mediators related to fibroblast-like synoviocyte activation, angiogenesis, articular cartilage degradation and osteoclast differentiation, inhibited HIF-1α expression and promoted anti-inflammatory mediator release in the joint tissues and serum of CIA mice.

Image Search Results

Cytokine array analysis revealed Pd1-dependent cytokines and chemokines in LPS-treated microglia . A , qPCR showing the decreased Pd1 mRNA in the microglia after Pd1 siRNA (20 nM and 50 nM) treatment for 48 h. All data are presented as mean ± SD. Statistical significance was indicated by ∗∗ p < 0.01, ∗∗∗ p < 0.001, as determined by one-way ANOVA. A : ANOVA, F (2, 12) = 15.32, p = 0.0005; NC siRNA versus Pd1 siRNA 20 nM, p = 0.0022; NC siRNA versus . Pd1 siRNA 50 nM, p = 0.0004; n = 5 wells per group). B , array membranes of protein expression among NC siRNA, NC siRNA + LPS (5 μg/ml), Pd1 siRNA (50 nM), and Pd1 siRNA (50 nM) + LPS (5 μg/ml) groups in culture medium for microglia. C , the expression of one colony-stimulating factor (G-CSF), one intercellular cell adhesion molecule (CD54), two inflammatory factors (IL-6 and TNF-α), and four chemokines (CXCL10, CCL12, CXCL9, and CCL5) significantly increased following LPS treatment, while the expression of one chemokine (CCL4) decreased. A significant increase in the expression of one inflammatory factor (TNF-α) and one chemokine (CXCL9) after treatment with Pd1 siRNA + LPS. All data are presented as mean ± SD. Statistical significance was indicated by ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001, as determined by two-way ANOVA. (Figure 6C: two-way ANOVA; G-CSF, NC siRNA versus Pd1 siRNA + LPS, p = 0.0016, Pd1 siRNA versus Pd1 siRNA + LPS, p = 0.0021; CD54, NC siRNA versus Pd1 siRNA, p = 0.0245, NC siRNA versus Pd1 siRNA + LPS, p = 0.0185; IL-6, NC siRNA versus NC siRNA + LPS, p < 0.0001, NC siRNA versus Pd1 siRNA + LPS, p < 0.0001, NC siRNA + LPS versus Pd1 siRNA, p < 0.0001; Pd1 siRNA versus Pd1 siRNA + LPS, p < 0.0001; CXCL10, NC siRNA versus NC siRNA + LPS, p < 0.0001, NC siRNA versus Pd1 siRNA, p < 0.0001, NC siRNA versus Pd1 siRNA + LPS, p < 0.0001, Pd1 siRNA versus Pd1 siRNA + LPS, p = 0.0481; CCL12, NC siRNA versus NC siRNA + LPS, p = 0.0012, NC siRNA versus Pd1 siRNA + LPS, p = 0.0017, NC siRNA + LPS versus Pd1 siRNA, p = 0.0012; Pd1 siRNA versus Pd1 siRNA + LPS, p = 0.0017; CXCL9, NC siRNA versus Pd1 siRNA + LPS, p < 0.0001, NC siRNA + LPS versus Pd1 siRNA + LPS, p = 0.0156, Pd1 siRNA versus Pd1 siRNA + LPS, p = 0.0145; CCL4, NC siRNA versus NC siRNA + LPS, p < 0.0001, NC siRNA versus Pd1 siRNA, p = 0.0062, NC siRNA versus Pd1 siRNA + LPS, p < 0.0001, Pd1 siRNA versus Pd1 siRNA + LPS, p = 0.0143; CXCL2, NC siRNA versus NC siRNA + LPS, p = 0.0121, NC siRNA versus Pd1 siRNA, p < 0.0001, NC siRNA versus Pd1 siRNA + LPS, p = 0.0017; CCL5, NC siRNA versus NC siRNA + LPS, p < 0.0001, NC siRNA versus Pd1 siRNA + LPS, p < 0.0001, NC siRNA + LPS versus Pd1 siRNA, p < 0.0001, Pd1 siRNA versus Pd1 siRNA + LPS, p < 0.0001; TNF-α, NC siRNA versus NC siRNA + LPS, p < 0.0001, NC siRNA versus Pd1 siRNA + LPS, p < 0.0001, NC siRNA + LPS versus Pd1 siRNA, p < 0.0001, NC siRNA + LPS versus Pd1 siRNA + LPS, p = 0.0442, Pd1 siRNA versus Pd1 siRNA + LPS, p < 0.0001; n = 3–4 separate experiments per group). G-CSF, granulocyte colony-stimulating factor; IL-6, interleukin-6; LPS, lipopolysaccharide; NC, negative control; Pd1, programmed cell death protein 1; qPCR, quantitative real-time PCR; siRNA, small interfering RNA; TNF-α, tumor necrosis factor-alpha.

Journal: The Journal of Biological Chemistry

Article Title: Effect of microglial Pd1 on glial scar formation after spinal cord injury in mice

doi: 10.1016/j.jbc.2025.108489

Figure Lengend Snippet: Cytokine array analysis revealed Pd1-dependent cytokines and chemokines in LPS-treated microglia . A , qPCR showing the decreased Pd1 mRNA in the microglia after Pd1 siRNA (20 nM and 50 nM) treatment for 48 h. All data are presented as mean ± SD. Statistical significance was indicated by ∗∗ p < 0.01, ∗∗∗ p < 0.001, as determined by one-way ANOVA. A : ANOVA, F (2, 12) = 15.32, p = 0.0005; NC siRNA versus Pd1 siRNA 20 nM, p = 0.0022; NC siRNA versus . Pd1 siRNA 50 nM, p = 0.0004; n = 5 wells per group). B , array membranes of protein expression among NC siRNA, NC siRNA + LPS (5 μg/ml), Pd1 siRNA (50 nM), and Pd1 siRNA (50 nM) + LPS (5 μg/ml) groups in culture medium for microglia. C , the expression of one colony-stimulating factor (G-CSF), one intercellular cell adhesion molecule (CD54), two inflammatory factors (IL-6 and TNF-α), and four chemokines (CXCL10, CCL12, CXCL9, and CCL5) significantly increased following LPS treatment, while the expression of one chemokine (CCL4) decreased. A significant increase in the expression of one inflammatory factor (TNF-α) and one chemokine (CXCL9) after treatment with Pd1 siRNA + LPS. All data are presented as mean ± SD. Statistical significance was indicated by ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001, as determined by two-way ANOVA. (Figure 6C: two-way ANOVA; G-CSF, NC siRNA versus Pd1 siRNA + LPS, p = 0.0016, Pd1 siRNA versus Pd1 siRNA + LPS, p = 0.0021; CD54, NC siRNA versus Pd1 siRNA, p = 0.0245, NC siRNA versus Pd1 siRNA + LPS, p = 0.0185; IL-6, NC siRNA versus NC siRNA + LPS, p < 0.0001, NC siRNA versus Pd1 siRNA + LPS, p < 0.0001, NC siRNA + LPS versus Pd1 siRNA, p < 0.0001; Pd1 siRNA versus Pd1 siRNA + LPS, p < 0.0001; CXCL10, NC siRNA versus NC siRNA + LPS, p < 0.0001, NC siRNA versus Pd1 siRNA, p < 0.0001, NC siRNA versus Pd1 siRNA + LPS, p < 0.0001, Pd1 siRNA versus Pd1 siRNA + LPS, p = 0.0481; CCL12, NC siRNA versus NC siRNA + LPS, p = 0.0012, NC siRNA versus Pd1 siRNA + LPS, p = 0.0017, NC siRNA + LPS versus Pd1 siRNA, p = 0.0012; Pd1 siRNA versus Pd1 siRNA + LPS, p = 0.0017; CXCL9, NC siRNA versus Pd1 siRNA + LPS, p < 0.0001, NC siRNA + LPS versus Pd1 siRNA + LPS, p = 0.0156, Pd1 siRNA versus Pd1 siRNA + LPS, p = 0.0145; CCL4, NC siRNA versus NC siRNA + LPS, p < 0.0001, NC siRNA versus Pd1 siRNA, p = 0.0062, NC siRNA versus Pd1 siRNA + LPS, p < 0.0001, Pd1 siRNA versus Pd1 siRNA + LPS, p = 0.0143; CXCL2, NC siRNA versus NC siRNA + LPS, p = 0.0121, NC siRNA versus Pd1 siRNA, p < 0.0001, NC siRNA versus Pd1 siRNA + LPS, p = 0.0017; CCL5, NC siRNA versus NC siRNA + LPS, p < 0.0001, NC siRNA versus Pd1 siRNA + LPS, p < 0.0001, NC siRNA + LPS versus Pd1 siRNA, p < 0.0001, Pd1 siRNA versus Pd1 siRNA + LPS, p < 0.0001; TNF-α, NC siRNA versus NC siRNA + LPS, p < 0.0001, NC siRNA versus Pd1 siRNA + LPS, p < 0.0001, NC siRNA + LPS versus Pd1 siRNA, p < 0.0001, NC siRNA + LPS versus Pd1 siRNA + LPS, p = 0.0442, Pd1 siRNA versus Pd1 siRNA + LPS, p < 0.0001; n = 3–4 separate experiments per group). G-CSF, granulocyte colony-stimulating factor; IL-6, interleukin-6; LPS, lipopolysaccharide; NC, negative control; Pd1, programmed cell death protein 1; qPCR, quantitative real-time PCR; siRNA, small interfering RNA; TNF-α, tumor necrosis factor-alpha.

Article Snippet: The levels of specific cytokines secreted by microglia were quantified using a mouse cytokine microarray kit (R&D Systems, ARY006).

Techniques: Expressing, Negative Control, Real-time Polymerase Chain Reaction, Small Interfering RNA

Figure 9. Cytokine and Chemokine expression in the OB and serum of WT, Cx3cr1/ and PLX5622-treated mice. (a) Cytokine and Chemokine microarray assay performed on total tissue lysates of OBs from WT, Cx3cr1 /, and PLX5622-terated mice. Expression levels are presented as arbitrary units, measured by densitometry. Using the Bonferroni procedure to correct for multiple comparisons, no significant changes were found between the expressed cytokines. n = 5, pooled in each group. Two-sample t-test. (b) Cytokine and Chemokine microarray assay performed on serum from WT, Cx3cr1/, and PLX5622-terated mice. Cytokine and Chemokine expression levels were measured by densitometry, and are presented as arbitrary units. Using the Bonferroni procedure to correct for multiple comparisons, significant changes were found in the following molecules: (C5a: WT vs PLX, t(2) =12.1, *p=0.0067; CXCL12: WT vs KO, t(2)=11.7, *p=0.007; TIMP1: WT vs PLX, t(2)=8.1, *p=0.014; ICAM1: WT vs KO, t(2)=10.4, *p=0.009). n = 5 pooled in each group. Two-sample t-test. DOI: https://doi.org/10.7554/eLife.30809.021 The following source data is available for figure 9:

Journal: eLife

Article Title: The role of microglia and their CX3CR1 signaling in adult neurogenesis in the olfactory bulb

doi: 10.7554/elife.30809

Figure Lengend Snippet: Figure 9. Cytokine and Chemokine expression in the OB and serum of WT, Cx3cr1/ and PLX5622-treated mice. (a) Cytokine and Chemokine microarray assay performed on total tissue lysates of OBs from WT, Cx3cr1 /, and PLX5622-terated mice. Expression levels are presented as arbitrary units, measured by densitometry. Using the Bonferroni procedure to correct for multiple comparisons, no significant changes were found between the expressed cytokines. n = 5, pooled in each group. Two-sample t-test. (b) Cytokine and Chemokine microarray assay performed on serum from WT, Cx3cr1/, and PLX5622-terated mice. Cytokine and Chemokine expression levels were measured by densitometry, and are presented as arbitrary units. Using the Bonferroni procedure to correct for multiple comparisons, significant changes were found in the following molecules: (C5a: WT vs PLX, t(2) =12.1, *p=0.0067; CXCL12: WT vs KO, t(2)=11.7, *p=0.007; TIMP1: WT vs PLX, t(2)=8.1, *p=0.014; ICAM1: WT vs KO, t(2)=10.4, *p=0.009). n = 5 pooled in each group. Two-sample t-test. DOI: https://doi.org/10.7554/eLife.30809.021 The following source data is available for figure 9:

Article Snippet: Shaker, Room temp recombinant DNA reagent Details for AAV1 Upenn, PA, U.S.A. Upenn viral core Cat. No. AV-1-PV3365 AAV1 under the CAG promoter, expressing TdTomato commercial assay or kit Cytokine and chemokine microarray assay R and D Systems, MN, U.S.A. Proteome Profiler Mouse Cytokine Array Kit (ARY006) c chemical compound, drug PLX5622 PLEXXIKON Inc., CA, U.S.A. AIN-76A Rodent Diet with PLX5622 1,200 mg PLX5622 (Free Base)/kg chemical compound, drug Control diet PLEXXIKON Inc., CA, U.S.A. AIN-76A Rodent Diet chemical compound, drug BrdU Sigma-Aldrich, MO, U.S.A. Sigma-Aldrich Cat. NO. B5002 10 mg/ml IP injection software, algorithm ImageJ/Figi University of Wisconsin, Madison, WI, U.S.A. (http://rsb.info.nih.gov/ij/), (http://fiji.sc/Fiji) (RRID:SCR_003070) software, algorithm Matlab Mathworks Inc., U.S.A. Matlab, R2014a (RRID:SCR_001622) software, algorithm SPSS I.B.M., U.S.A. SPSS, Version 19 (RRID:SCR_002865)

Techniques: Expressing, Microarray